LOCUS       40924_44309        6334 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pTR_KJUMPS DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Kasai Y, Matsuzaki K, Ikeda F, Yoshimitsu Y, Harayama S.
  TITLE     Precise excision of a selectable marker gene in transgenic 
            Pseudococcomyxa strains by the piggyBac transposase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Kasai Y, Harayama S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2017) Contact:Yuki Kasai Chuo University, Research
            and Development Initiative; 1-13-27 Kasuga, Bunkyo-ku, Tokyo 
            112-8551, Japan
REFERENCE   3  (bases 1 to 6334)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6334)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUN-2017) Contact:Yuki Kasai Chuo University, Research and 
            Development Initiative; 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, 
            Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..858
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1032..1620
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1908..1929
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1944..1974
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2006..2022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  2513..2547
                     /mobile_element_type="transposon"
                     /label=transposon
                     /note="piggyBac transposon 5' terminal repeat"
     regulatory      2548..3195
                     /gene="EF1a"
                     /note="Includes promoter region of Pseudococcomyxa sp.
                     strain KJ nuclear gene EF1a for elongation factor 1 alpha 
                     subunit"
                     /regulatory_class="promoter"
     gene            2548..3195
                     /gene="EF1a"
                     /label=EF1a
     regulatory      3190..3199
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3196..4701
                     /codon_start=1
                     /gene="UMPS"
                     /product="uridine monophosphate synthase"
                     /label=UMPS
                     /protein_id="BAY00745.1"
                     /translation="MATSTPSVATAAELKPKEGPITSQEFEELVLRLHEIEAVKFGNFK
                     LKSGLMSPIYIDLRVIVSYPDVLRRVAEVMWHQVSGAGFDVMCGVPYTALPIATCMSLL
                     HGTPMLMRRKEVKEYGTKKAIEGAFKKGQTCLIVEDLVTSGASVMETVEPLEVEGLKVA
                     DVVVLIDREQGGAARMASNGLRLHSAFTLSFIIKTLQAHGLVSAEVADSVAAFIAANQT
                     FSPSAAAAPTPASAPPAQPKRLPFGERAALCQNAAGRKLLELMARKRTNLAVAADVATV
                     EEMLRIADAAGPHIAVFKTHVDIFDEWDDGIAAQLRHLADKHEFLIFEDRKFADIGNTV
                     VSQYGGGIYKIADWSDITNAHLVPGPGIIDGLRKVGQEKGRGLLLLAEMSSKGALATGA
                     YTEKVAEAAAANQDFVMGFICQSPAKWATSVPPGLVHMTPGVQLASGSDALGQQYNTPA
                     SVIGQGGSDVIIVGRGIIKAADPAAAAAQYREAGWAAYEATLA"
     gene            3196..4701
                     /gene="UMPS"
                     /label=UMPS
     regulatory      4702..5398
                     /gene="EF1a"
                     /note="Includes terminator region of Pseudococcomyxa sp.
                     strain KJ nuclear gene EF1a for elongation factor alpha 
                     subunit"
                     /regulatory_class="terminator"
     gene            4702..5398
                     /gene="EF1a"
                     /label=EF1a
     mobile_element  5399..5461
                     /mobile_element_type="transposon"
                     /label=transposon
                     /note="piggyBac transposon 3' inverted terminal repeat"
     primer_bind     complement(5591..5607)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5749..6204
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6230..6334
                     /label=AmpR promoter
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     2041 gcccgccggg taactcacgg ggtatccatg tccatttctg cggcatccag ccaggatacc
     2101 cgtcctcgct gacgtaatat cccagcgccg caccgctgtc attaatctgc acaccggcac
     2161 ggcagttccg gctgtcgccg gtattgttcg ggttgctgat gcgcttcggg ctgaccatcc
     2221 ggaactgtgt ccggaaaagc cgcgacgaac tggtatccca ggtggcctga acgaacagtt
     2281 caccgttaaa ggcgtgcatg gccacacctt cccgaatcat catggtaaac gtgcgttttc
     2341 gctcaacgtc aatgcagcag cagtcatcct cggcaaactc tttccatgcc gcttcaacct
     2401 cgcgggaaaa ggcacgggct tcttcctccc cgatgcccag atagcgccag cttgggcgat
     2461 gactgagccg gaaaaaagac ccgacgatat gatcctgatg cagctagatt aaccctagaa
     2521 agatagtctg cgtaaaattg acgcatgtgt aatggccata tcgaagcact gttctggaaa
     2581 tctattcaag tttgaggaca aaaatgcatc attagaccgc agtctgacgt gccctgaagc
     2641 ctgagcctga catgtgatgc ctcgtcgtgc aacaaatcgt ggcgggtgaa cagcgctatt
     2701 taaaagaaga gccagctcaa actgactcct gtttatagga tattcatgat aaggaatcag
     2761 aatcactttc cacacaccaa agtgatctag tactgccgca agatcaaaac tcgtggaaga
     2821 gtgcaagacc gcgcttgcaa agaatcacaa tagcccaatg ctttcagtag cagacaactc
     2881 ttaagtgcta atttgctgcc cacaatttcc ggggctggag tttcagagca ttccagacgc
     2941 cacttgccag atttggctcc atcagggaac tggaacatcg agagagctct ctgtcaagcc
     3001 gcctctcctg cccaccagca aaagtatcct ttggcatcca acctttggta cctctgtcac
     3061 aggtgtggtt gctttccgag ccgtctaacg tcttttctgt ctgaaagcga ggtggttttt
     3121 gtgttgcgct tgccacagaa aggcttggtg caagtgatct gaccagctta tctgtttgca
     3181 ggaaaactcg ccaccatggc aacttcaact ccctcagtgg ccacagctgc ggagctgaaa
     3241 ccaaaggaag ggcccatcac gtcacaggag tttgaagagc tcgtgctgcg tttgcatgaa
     3301 atcgaggccg tcaaatttgg caacttcaag ctcaagagcg ggctcatgtc gccgatctac
     3361 atcgatctgc gcgtcattgt gtcctatccg gacgtcctgc gcagggtggc agaggtgatg
     3421 tggcatcagg tgtcgggtgc tggctttgac gtgatgtgcg gcgtgccata cacggcgctg
     3481 cccatagcga catgcatgtc gctgctgcac gggacgccca tgctgatgcg gcgcaaggag
     3541 gtgaaggagt acggcaccaa gaaggccatc gagggcgcct tcaagaaggg ccagacctgc
     3601 ctcatcgtgg aggatctggt caccagcggc gcctccgtca tggagactgt ggagcccctc
     3661 gaggtggagg ggctgaaggt ggcggatgtg gtggtgctca tagaccggga gcagggcggc
     3721 gcggcgcgca tggccagcaa cgggctgcga ctgcactccg ccttcacgct gtccttcatc
     3781 atcaagacgc tgcaggcgca cggcctcgtc agcgccgagg tggccgacag cgtcgctgcc
     3841 ttcatagccg ccaaccaaac cttcagcccc tctgcagcag ccgcccccac acccgcgtct
     3901 gcccctcctg cgcagcccaa acggctgcca tttggggagc gcgcggcgct gtgtcagaac
     3961 gcagccggca ggaagctgct ggagctgatg gcgcgcaagc ggaccaacct ggcggtggcg
     4021 gcggacgtgg cgacggtgga ggagatgctg cgcatcgcgg acgcggccgg gccgcacatc
     4081 gccgtcttca agacccacgt ggacattttt gatgagtggg atgatggcat cgccgcccag
     4141 ctgcgccatc tggccgacaa gcatgagttc ttgatattcg aggacaggaa gtttgcggac
     4201 atcggtaaca cagtggtgtc gcagtacggc ggaggcatct acaagatcgc cgactggtca
     4261 gacatcacca atgcacacct ggtcccgggc ccaggcatca tcgatggtct ccgcaaggtg
     4321 ggccaggaga aggggcgagg cctgctgctg ctggctgaga tgagctccaa gggagctctt
     4381 gccacagggg cctacaccga gaaggttgct gaagcggcag ctgccaacca ggactttgtc
     4441 atgggcttca tctgccagtc accagccaag tgggccactt cagtgcctcc aggtctggtg
     4501 cacatgacgc cgggtgtgca gctggcgtct gggagcgatg cactggggca gcagtacaac
     4561 acacctgctt ctgtcatcgg ccagggcggc agcgatgtca tcattgtggg gagagggatc
     4621 atcaaggcgg cagaccccgc tgcagcggcc gcacagtaca gagaagccgg ctgggccgcc
     4681 tatgaggcca cgctggcatg accatggccg ctgagagctg cgtgaggatc tggggtagga
     4741 gctgtgcagc ctctgccgct gctggcccct gctgttgtac acaagtgccg cccgcccagc
     4801 gggaggtgct cggaccctgc tggcatgcct tggttgctag gcaggctcag aactgggtgc
     4861 tggactttag gtggcgtggc ttcacgcagc tccttgtctg agcagggctt ctgccctgcg
     4921 gcgtgtgcgc ctttctgtgt gcatcccccg gatctctccg ttgcagtact tgtcagcagt
     4981 ctctcttttc cctgtcagta ggataggcca gagctactcc ggcttccatc aaacattttg
     5041 gaacttttca tcctgcgctt cttgtcttca cgatcctagg atgtgtggtg gggatgatac
     5101 gctttgtatt cacacactcg agttcaacaa ctttcttagg gtgttcaact ggggaggggc
     5161 attgtgacca tcctgacaga catacatctc acacgtcaaa gagggcttga aaaggagtgc
     5221 atatgcatgt gcatatcctg tggttgttga gactacagga agagtgtaaa gggttcaact
     5281 cacaagaaaa gtttctgtgg aagcagtgcg tcagtgcatg catggactga gttaagagcc
     5341 gcaggttgtc acacacttca catcctgttg tttagaagca tcatgtttgc aagcactcca
     5401 tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg
     5461 gttaatctag ctgcgtgttc tgcagcgtgt cgagcatctt catctgctcc atcacgctgt
     5521 aaaacacatt tgcaccgcga gtctgcccgt cctccacggg ttcaaaaacg tgaatgaacg
     5581 aggcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     5641 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     5701 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     5761 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5821 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5881 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5941 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6001 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6061 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6121 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     6181 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6241 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6301 ataaatgctt caataatatt gaaaaaggaa gagt
//
